5-Aminoimidazole-4-carboxamide ribonucleotide or AICAR is an analogue of adenosine monophosphate. The AICAR peptide is a cell penetrable activator of AMP-activated protein kinase (AMPK), a master regulator of metabolic rate that is switched on in times of limited energy availability and works to inhibit anabolic metabolism. Inside the body, activation of AMPK with AICAR imitates that the body is exercising and this triggers insulin-independent glucose uptake by muscle cells. This makes AICAR important for improving endurance and burning stored fat. AICAR was shown to stimulate fat breakdown decades ago and causes cells to move their fat stores into small organelles called mitochondria, where they are broken down to release their stored energy. Animal studies have shown that AICAR can potently activate many metabolic genes to increase exercise endurance. Interestingly, the compound also increased the exercise endurance in sedentary animals. In animals, the compound has also been shown to reduce tissue inflammation and promote the healing of injured and damaged tissue. The use of AICAR in human trials has shown that it can improve glucose regulation in diabetic patients by changing how their bodies respond to glucose, providing evidence that AICAR is useful in prevention and treatment of type II diabetes due to its glucose regulatory properties. Additionally, AICAR has been shown to protecting against cardiac ischemic injury and to improve myocardial protection during heart bypass surgery. Evidence has also shown that AICAR stimulation closely mimics the metabolic changes seen in contracting muscles, even without prior exercise. AICAR also acts as an anti-inflammatory and reduces inflammation by disrupting signaling molecules that cause cells to become activated in mounting an immune response. In conclusion, AICAR is useful for tissue healing, burning fat, stimulating muscle contraction and for reducing inflammation. AICAR is fast acting,but has a short-half life and is rapidly eliminated by the body. AICAR cantherefore be dosed daily. The recommended AICAR dose is 25 mg orally either once or twice perday.